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KMID : 1234420110390030252
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Korean Journal of Microbiololgy and Biotechnology
2011 Volume.39 No. 3 p.252 ~ p.258
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Production and Properties of Hemicellulases by a Cellulosimicrobium sp. Isolate
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Yoon Ki-Hong
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Abstract
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A bacterial strain capable of hydrolyzing xylan and locust bean gum (LBG) was isolated from farm soil through an enrichment culture using a mixture of palm kernel meal (PKM) and wheat bran as the carbon source. Nucleotide sequencing of 16S rDNA, amplified from the isolate YB-1107, showed a high similarity with those of genus Cellulosimicrobium strains. Xylanase productivity was increased when Cellulosimicrobium sp. YB-1107 was grown in the presence of wheat bran or oat spelt xylan, while mannanase productivity was increased drastically when grown in the presence of PKM or LBG. Particularly, maximum mannanase and xylanase activities were obtained in a culture filtrate of media containing 0.7% PKM or 1% wheat bran, respectively. Both enzyme activities were produced at the stationary growth phase. Mannanase from the culture filtrate showed the highest activity at 55oC and a pH of 6.5. Xylanase activity was optimal at 65oC and a pH of 5.5. The predominant products resulting from mannanase or xylanase hydrolysis were oligosaccharides for LBG or xylan, respectively. In addition, the enzymes could hydrolyze wheat bran and rice bran into oligosaccharides.
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KEYWORD
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Cellulosimicrobium sp., mannanase, xylanase, productivity
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